in-house matlab program 2021a (MathWorks Inc)
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Figures S1–S3 ; In House Matlab Program 2021a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia"
Article Title: CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia
Journal: Cell Reports Methods
doi: 10.1016/j.crmeth.2023.100597
Figures S1–S3 ; Figure Legend Snippet: CartoCell pipeline for high-content epithelial cysts segmentation Phase 1: the “high-resolution raw images” consist of confocal z-stack images, where the cell membrane is stained. These images are segmented and proofread using LimeSeg and a custom MATLAB code for curation to obtain the “high-resolution label images” . Together, the raw and the label images encompass the “training high-resolution dataset.” Number of samples = 21. Phase 2: the “training high-resolution dataset” is down-sampled to obtain the “training down-sampled dataset,” which is the training set for the “model M1.” Phase 3: low-resolution images are obtained from confocal z-stack images, stained in a similar way to phase 1. Number of samples = 293. Scale bar, 100 μm. Next, the “work-flow M1” is applied: inference using “model M1” and subsequent post-processing to obtain individual cell instance predictions and cell masks, followed by the 3D Voronoi algorithm to guarantee that predicted cells remain in close contact. As a result, the “low-resolution label images” are generated. Phase 4: training of the “model M2” on the large “training low-resolution dataset.” Number of samples = 314. Phase 5: high-content segmentation of new low-resolution images (unseen by the pipeline) using the “work-flow M2,” which is equivalent to the “work-flow M1” but using the “model M2.” See also
Techniques Used: Membrane, Staining, Generated
Figure Legend Snippet:
Techniques Used: Recombinant, Software